Journal: Journal of Clinical & Cellular Immunology

Article Title: Identification of Thyroglobulin and its Isoforms as Target Antigens for IgG4 Thyroiditis

doi: 10.4172/2155-9899.1000568

Figure Lengend Snippet: Figure 1: Representative histopathological and immunohistochemical findings in a patient with IgG4 thyroiditis; (A) Diffuse lymphocytic infiltration and fibrosis were observed in thyroid tissue; (B) Lymphoid follicle (germinal center) formation, follicular cell degeneration and eosinophilic change of thyroid follicular epithelial cells were also observed; (C, D) IgG-positive plasma cells and (E, F) IgG4-positive plasma cells were observed in the lymphocyte-infiltrated peripheral lesion, and fibrotic area (C, E) and also highly in germinal center (D, F). Magnification: hematoxylin and eosin, HE-stained, 100 (A, B), immunostaining of IgG and IgG4, 400 (C, D, E, F); Diagnostic criteria: IgG4 thyroiditis was diagnosed according to the condition when the number of IgG4-positive plasma cells are more than 20/HPF and the ratio of IgG4-positive cells to IgG-positive cells (IgG4+/IgG+cell ratio) exceeded 30%.

Article Snippet: Immunostaining for IgG4 (mouse monoclonal, HP6025, Southern Biotech, Chicago, USA; dilution 1:400) and IgG (rabbit polyclonal, Agilent, California, USA) were performed using EnVision system (Agilent, California, USA).

Techniques: Immunohistochemical staining, Clinical Proteomics, Staining, Immunostaining, Diagnostic Assay

Journal: Journal of Clinical & Cellular Immunology

Article Title: Identification of Thyroglobulin and its Isoforms as Target Antigens for IgG4 Thyroiditis

doi: 10.4172/2155-9899.1000568

Figure Lengend Snippet: Figure 2: Identification of the target antigens recognized by serum IgG4 of IgG4 thyroiditis patients by MALDI-TOF/MS analysis; (A) Five reacting bands were able to be distinguished from other bands. The western blotting was performed using first antibody as serum of an IgG4 thyroiditis patient No.3 with high serum IgG4 level; (B) Protein scores by MALDI-TOF/ MS analysis (1a, 2a, 3a, 4a, 5a) and matched sequence were identified by MASCOT search of each band (1b, 2b, 3b, 4b, 5b). Predicted protein from five bands was thyroglobulin isoform X2. Its sequence abd matched regions of each peptide are shown in white letters on a black background; (C) Total IgG, IgG1and IgG4 antibodies against extraction of the thyroid antigens were observed in IgG4 thyroiditis patients No. 4, 5, 7 and 10; (D) The presence of IgG4 antibodies to the recombinant thyroglobulin protein (MW=37.84 kDa) were confirmed in serum of No.3 and No.7 patients (black arrow). Abbreviation: M=marker; S=sample; r-Tg=recombinant thyroglobulin.

Article Snippet: Immunostaining for IgG4 (mouse monoclonal, HP6025, Southern Biotech, Chicago, USA; dilution 1:400) and IgG (rabbit polyclonal, Agilent, California, USA) were performed using EnVision system (Agilent, California, USA).

Techniques: Western Blot, Sequencing, Extraction, Recombinant, Marker

Journal: Molecular Therapy

Article Title: C3d-Targeted factor H inhibits tissue complement in disease models and reduces glomerular injury without affecting circulating complement

doi: 10.1016/j.ymthe.2024.02.001

Figure Lengend Snippet: C3d-mAb-2fH binding affinity rises with increased C3d density

Article Snippet: Rodent C3d was stained using a human anti-C3d IgG4 (clone 3d8b, ADX-086) followed by a secondary Alexa Fluor 647-conjugated mouse anti-human IgG4 pFc′ (Southern Biotech 9190-31 clone HP6023) or Alexa Fluor 488-conjugated mouse anti-human IgG4 pFc′ (Southern Biotech 9190-30 clone HP6023).

Techniques: Binding Assay

Journal: Molecular Therapy

Article Title: C3d-Targeted factor H inhibits tissue complement in disease models and reduces glomerular injury without affecting circulating complement

doi: 10.1016/j.ymthe.2024.02.001

Figure Lengend Snippet: In vitro complement inhibition by C3d-mAb-fH fusion proteins

Article Snippet: Rodent C3d was stained using a human anti-C3d IgG4 (clone 3d8b, ADX-086) followed by a secondary Alexa Fluor 647-conjugated mouse anti-human IgG4 pFc′ (Southern Biotech 9190-31 clone HP6023) or Alexa Fluor 488-conjugated mouse anti-human IgG4 pFc′ (Southern Biotech 9190-30 clone HP6023).

Techniques: In Vitro, Inhibition, Activity Assay

Journal: PLoS ONE

Article Title: Evaluation of Naturally Acquired IgG Antibodies to a Chimeric and Non-Chimeric Recombinant Species of Plasmodium vivax Reticulocyte Binding Protein-1: Lack of Association with HLA-DRB1*/DQB1* in Malaria Exposed Individuals from the Brazilian Amazon

doi: 10.1371/journal.pone.0105828

Figure Lengend Snippet: (A) Frequency of IgG responders in the studied population to the recombinant chimeric protein PvRMC-RBP1 and non-chimeric PvRBP1 23-751 . The Chi squared test for proportions analyses was performed to determine statistical differences. The frequency of IgG responders to PvRBP1 23-751 was significantly higher when compared to PvRMC-RBP1. (B) The median of the IgG reactivity index against both antigens was not significant (Mann Whitney test, p = 0.6833). (C) Reactivity indexes of IgG antibodies against PvRMC-RBP1 and PvRBP1 23-751 showed significant correlation by nonparametric Spearman test (r = 0.5768; p<0.0001).

Article Snippet: IgG subclasses were determined in individual responders by ELISA as described above where the following peroxidase conjugated monoclonal mouse anti-human antibodies were used: Mouse Anti-Human IgG1 (hinge)-HRP (clone HP6001, Southern Biotechnology), Mouse Anti-Human IgG2 (Fc)-HRP (clone HP6002, Southern Biotechnology), Mouse Anti-Human IgG3 (hinge)-HRP (clone HP6050, Southern Biotechnology), Mouse Anti-Human IgG4 (Fc)-HRP (clone HP6023, Southern Biotechnology), all diluted by 1∶1000.

Techniques: Recombinant, MANN-WHITNEY

Journal: PLoS ONE

Article Title: Evaluation of Naturally Acquired IgG Antibodies to a Chimeric and Non-Chimeric Recombinant Species of Plasmodium vivax Reticulocyte Binding Protein-1: Lack of Association with HLA-DRB1*/DQB1* in Malaria Exposed Individuals from the Brazilian Amazon

doi: 10.1371/journal.pone.0105828

Figure Lengend Snippet: The median of reactivity index of IgG to PvRMC-RBP1 presented no significant difference between before and after absorption (p = 0.2525).

Article Snippet: IgG subclasses were determined in individual responders by ELISA as described above where the following peroxidase conjugated monoclonal mouse anti-human antibodies were used: Mouse Anti-Human IgG1 (hinge)-HRP (clone HP6001, Southern Biotechnology), Mouse Anti-Human IgG2 (Fc)-HRP (clone HP6002, Southern Biotechnology), Mouse Anti-Human IgG3 (hinge)-HRP (clone HP6050, Southern Biotechnology), Mouse Anti-Human IgG4 (Fc)-HRP (clone HP6023, Southern Biotechnology), all diluted by 1∶1000.

Techniques:

Journal: PLoS ONE

Article Title: Evaluation of Naturally Acquired IgG Antibodies to a Chimeric and Non-Chimeric Recombinant Species of Plasmodium vivax Reticulocyte Binding Protein-1: Lack of Association with HLA-DRB1*/DQB1* in Malaria Exposed Individuals from the Brazilian Amazon

doi: 10.1371/journal.pone.0105828

Figure Lengend Snippet: (A) Frequency of IgG subclass responders to the recombinant chimeric protein PvRMC-RBP1 and non-chimeric PvRBP1 23-751 among the IgG responders. The Chi squared test for proportions analyses was performed to determine that the frequency of IgG1 responders was significantly higher when compared with all others IgG subclasses. (B) The medians of reactivity indexes of IgG1 subclass against both antigens tested were also higher than IgG2, IgG3 and IgG4 by Mann Whitney test. * The frequency of IgG1 responders to PvRMC-RBP1 was significantly higher when compared with IgG2 (X 2 = 90.4; p = 0.0019), IgG3 (X 2 = 98.6; p<0.0001) and IgG4 (X 2 = 108.5; p<0.0001). # The frequency of IgG1 responders to PvRBP1 23-751 was significantly higher when compared with IgG2 (X 2 = 73.6; p<0.0001), IgG3 (X 2 = 54.6; p<0.0001) and IgG4 (X 2 = 55.9; p<0.0001). § The median of reactivity index of IgG1 to PvRMC-RBP1 was significantly higher than IgG2 (p<0.0001), IgG3 (p<0.0001) and IgG4 (p<0.0001) by Mann Whitney test. + The median of reactivity index of IgG1 to PvRBP1 23-751 was significantly higher than IgG2 (p<0.0001), IgG3 (p<0.0001) and IgG4 (p<0.0001) by Mann Whitney test.

Article Snippet: IgG subclasses were determined in individual responders by ELISA as described above where the following peroxidase conjugated monoclonal mouse anti-human antibodies were used: Mouse Anti-Human IgG1 (hinge)-HRP (clone HP6001, Southern Biotechnology), Mouse Anti-Human IgG2 (Fc)-HRP (clone HP6002, Southern Biotechnology), Mouse Anti-Human IgG3 (hinge)-HRP (clone HP6050, Southern Biotechnology), Mouse Anti-Human IgG4 (Fc)-HRP (clone HP6023, Southern Biotechnology), all diluted by 1∶1000.

Techniques: Recombinant, MANN-WHITNEY

Journal: PLoS ONE

Article Title: Evaluation of Naturally Acquired IgG Antibodies to a Chimeric and Non-Chimeric Recombinant Species of Plasmodium vivax Reticulocyte Binding Protein-1: Lack of Association with HLA-DRB1*/DQB1* in Malaria Exposed Individuals from the Brazilian Amazon

doi: 10.1371/journal.pone.0105828

Figure Lengend Snippet: (A) Spearman's rank correlation between age and IgG reactivity index against the chimeric recombinant protein in a population naturally exposed to malaria (r = 0.3613; p<0.0001). (B) Spearman's rank correlation between time of residence in malaria endemic area and IgG reactivity index against the chimeric recombinant protein in a population naturally exposed to malaria (r = 0.2781; p<0.0001).

Article Snippet: IgG subclasses were determined in individual responders by ELISA as described above where the following peroxidase conjugated monoclonal mouse anti-human antibodies were used: Mouse Anti-Human IgG1 (hinge)-HRP (clone HP6001, Southern Biotechnology), Mouse Anti-Human IgG2 (Fc)-HRP (clone HP6002, Southern Biotechnology), Mouse Anti-Human IgG3 (hinge)-HRP (clone HP6050, Southern Biotechnology), Mouse Anti-Human IgG4 (Fc)-HRP (clone HP6023, Southern Biotechnology), all diluted by 1∶1000.

Techniques: Recombinant

Journal: Cell Reports Medicine

Article Title: Multi-omic profiling reveals the endogenous and neoplastic responses to immunotherapies in cutaneous T cell lymphoma

doi: 10.1016/j.xcrm.2024.101527

Figure Lengend Snippet:

Article Snippet: Mouse Anti-Human IgG4 Fc-UNLB (HP6025) , SouthernBiotech , CAT#9200-01; RRID:AB_2796689.

Techniques: Recombinant, Marker, Purification, Blocking Assay, Antibody Labeling, Software